Host-targeted Therapeutics

Walker, A. C., Bhargava, R., Brust, A. S., Owji A. A., Czyz, D. M. (2022). Time-off-pick assay to measure Caenorhabditis elegans motitlity. Bio-protocols. In review

Walker, A. C., Bhargava, R., Vaziriyan-Sani, A. S., Brust, A. S. and Czyz, D. M. (2022). Quantification of Bacterial Loads in Caenorhabditis elegans. Bio-protocol 12(2): e4291. DOI: 10.21769/BioProtoc.4291.

Czyz DM. Targeting the human gut microbiota as an intervention strategy in protein conformational diseases. Neural Regeneration Research. Vol 17. In Press

Daga, A., Nguyen, O.T., Moothedan, E. Czyz, D.M., Faldu, A., Ham, T., Goyal, A., Motwani, K., Feller, D.B. Antibiotic prescribing patterns for acute respiratory infections in a free clinic network: a pooled cross-sectional studyDrugs Ther Perspect (2021).

Zhou H, Gebhardt MJ, Czyz DM, Yao Y, Shuman HA. The gigA/gigB Genes Regulate the Growth, Stress Response, and Virulence of Acinetobacter baumannii ATCC 17978 Strain. Front Microbiol. 2021;12:723949. Published 2021 Aug 4. doi:10.3389/fmicb.2021.723949

Vaziriyan-Sani AS, Handy RD, Walker AC, Pagolu CN, Enslow SM, Czyż DM. Automating Aggregate Quantification in Caenorhabditis elegansJ Vis Exp. 2021;(176):10.3791/62997. Published 2021 Oct 14. doi:10.3791/62997

Yersinia pseudotuberculosis YopJ Limits Macrophage Response by Downregulating COX-2-Mediated Biosynthesis of PGE2 in a MAPK/ERK-Dependent Manner

Sheppe AEF, Santelices J, Czyz DM, Edelmann MJ. Microbiol Spectr. 2021 Jul 28:e0049621. doi: 10.1128/Spectrum.00496-21.

Epub ahead of print. PMID: 34319170.

The potential impact of the COVID-19 pandemic on antimicrobial resistance and antibiotic stewardship

Subramanya SH, Czyż DM, Acharya KP, Humphreys H.

Virusdisease. 2021 May 25:1-8. doi: 10.1007/s13337-021-00695-2. Epub ahead of print. PMID: 34056051; PMCID: PMC8145182.

Colonization of the Caenorhabditis elegans gut with human enteric bacterial pathogens leads to proteostasis disruption that is rescued by butyrate.

Walker AC, Bhargava R, Vaziriyan-Sani AS, Pourciau C, Donahue ET, Dove AS, Gebhardt MJ, Ellward GL, Romeo T, Czyż DM.

PLoS Pathog. 2021 May 6;17(5):e1009510. doi: 10.1371/journal.ppat.1009510. PMID: 33956916; PMCID: PMC8101752.

GigC, a LysR-family Transcription Regulator is required for cysteine metabolism and virulence in Acinetobacter baumannii

Gebhardt MJ, Czyz DM, Singh S, Zurawski DV, Becker L, Shuman HA.

Infect Immun. 2020 Dec 15;89(1):e00180-20. doi: 10.1128/IAI.00180-20. PMID: 33077621; PMCID: PMC7927930.

Metabolic regulation of gene expression by histone lactylation.

Zhang D, Tang Z, Huang H, Zhou G, Cui C, Weng Y, Liu W, Kim S, Lee S, Perez-Neut M, Ding J, Czyz D, Hu R, Ye Z, He M, Zheng YG, Shuman HA, Dai L, Ren B, Roeder RG, Becker L, Zhao Y.  

Nature2019 Oct;574(7779):575-580doi: 10.1038/s41586-019-1678-1. Epub 2019 Oct 23. PubMed PMID: 31645732.

Brucella periplasmic protein EipB is a molecular determinant of cell envelope integrity and virulence

Julien Herrou, Jonathan W Willett, Aretha Fiebig, Daniel M Czyż, Jason X Cheng, Eveline Ultee, Ariane Briegel, Lance Bigelow, Gyorgy Babnigg, Youngchang Kim, Sean Crosson

J Bacteriol 201:e00134-19, 2019

Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus

Julien Herrou, Jonathan W Willett, Aretha Fiebig, Lydia M Varesio, Daniel M Czyż, Jason X Cheng, Eveline Ultee, Ariane Briegel, Lance Bigelow, Gyorgy Babnigg, Youngchang Kim, Sean Crosson

Molecular Microbiology. 2019 Mar;111(3):637-661. doi: 10.1111/mmi.14178. Epub 2019 Jan 21.

Brucella EipB is periplasmic β-spiral protein required for envelope stress resistance and infection

Julien Herrou, Jonathan Willett, Aretha Fiebig, Daniel Czyz, Jason Cheng, Eveline Ultee, Ariane Briegel, Lance Bigelow, Gyorgy Babnigg, Youngchang Kim, Sean Crosson

bioRxiv. 2019. Cold Spring Harbor

Gallium disrupts bacterial iron metabolism and has therapeutic effects in mice and humans with lung infections.

Goss CH, Kaneko Y, Khuu L, Anderson GD, Ravishankar S, Aitken ML, Lechtzin N, Zhou G, Czyz DM, McLean K, Olakanmi O, Shuman HA, Teresi M, Wilhelm E, Caldwell E, Salipante SJ, Hornick DB, Siehnel RJ, Becker L, Britigan BE, Singh PK.

Sci Transl Med. 2018 Sep 26;10(460). pii: eaat7520. doi: 10.1126/scitranslmed.aat7520.

PMID: 30257953

Obesity and Insulin Resistance Promote Atherosclerosis through an IFNγ-Regulated Macrophage Protein Network.

Reardon CA, Lingaraju A, Schoenfelt KQ, Zhou G, Cui C, Jacobs-El H, Babenko I, Hoofnagle A, CzyzD, Shuman H, Vaisar T, Becker L.

Cell Rep. 2018 Jun 5;23(10):3021-3030. doi: 10.1016/j.celrep.2018.05.010.

PMID: 29874587

Molecular control of gene expression by Brucella BaaR, an IclR-type transcriptional repressor.

Herrou J, Czyż DM, Fiebig A, Willett JW, Kim Y, Wu R, Babnigg G, Crosson S.

J Biol Chem. 2018 May 11;293(19):7437-7456. doi: 10.1074/jbc.RA118.002045. Epub 2018 Mar 22.

PMID: 29567835

Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen.

Czyż DM, Willett JW, Crosson S.

J Bacteriol. 2017 Jul 11;199(15). pii: e00227-17. doi: 10.1128/JB.00227-17. Print 2017 Aug 1.

PMID: 2855929

Conserved ABC Transport System Regulated by the General Stress Response Pathways of Alpha- and Gammaproteobacteria.

Herrou J, Willett JW, Czyż DM, Babnigg G, Kim Y, Crosson S.

J Bacteriol. 2017 Feb 14;199(5). pii: e00746-16. doi: 10.1128/JB.00746-16. Print 2017 Mar 1.

PMID: 27994018

A dual-targeting approach to inhibit Brucella abortus replication in human cells.

Czyż DM, Jain-Gupta N, Shuman HA, Crosson S.

Sci Rep. 2016 Oct 21;6:35835. doi: 10.1038/srep35835.

PMID: 27767061

Brucella abortus ΔrpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis.

Willett JW, Herrou J, Czyz DM, Cheng JX, Crosson S.

Vaccine. 2016 Sep 30;34(42):5073-5081. doi: 10.1016/j.vaccine.2016.08.076. Epub 2016 Aug 31.

PMID: 27591954

WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System.

Herrou J, Czyż DM, Willett JW, Kim HS, Chhor G, Babnigg G, Kim Y, Crosson S.

J Bacteriol. 2016 Mar 31;198(8):1281-93. doi: 10.1128/JB.00982-15. Print 2016 Apr.

PMID: 26858101

Host-directed antimicrobial drugs with broad-spectrum efficacy against intracellular bacterial pathogens.

Czyż DM, Potluri LP, Jain-Gupta N, Riley SP, Martinez JJ, Steck TL, Crosson S, Shuman HA, Gabay JE.

MBio. 2014 Jul 29;5(4):e01534-14. doi: 10.1128/mBio.01534-14.

PMID: 25073644

Identification of a tissue-selective heat shock response regulatory network.

Guisbert E, Czyz DM, Richter K, McMullen PD, Morimoto RI.

PLoS Genet. 2013 Apr;9(4):e1003466. doi: 10.1371/journal.pgen.1003466. Epub 2013 Apr 18.

PMID: 23637632